2011

Modulation of Ligand-Field Parameters by Heme Ruffling in Cytochromes c Revealed by EPR Spectroscopy, Mehmet Can, Giorgio Zoppellaro, K. Kristoffer Andersson, Kara L. Bren, Inorg. Chem.2011, 50, 12018-12024. DOI: 10.1021/ic201479q

Methionine Ligand Lability in Bacterial Monoheme Cytochromes c: An Electrochemical Study, Benjamin D. Levin, Mehmet Can, Sarah E. J. Bowman, Kara L. Bren, Sean J. Elliott, J. Phys. Chem. B2011, 115, 11718-11726. DOI: 10.1021/jp203292h

Comparing substrate specificity between cytochrome c maturation and cytochrome c heme lyase systems for cytochrome c biogenesis, Jesse G. Kleingardner, Kara L. Bren, Metallomics, 2011, 3, 396-403. DOI: 10.1039/c0mt00086h

Temperature Dependent Equilibrium Native to Unfolded Protein Dynamics and Properties Observed with IR Absorption and 2D IR Vibrational Echo Experiments, Jean K. Chung, Megan C. Thielges, Sarah E. J. Bowman, Kara L. Bren, M. D. Fayer, J. Am. Chem. Soc. 2011, 133, 6681-6691. DOI: 10.1021/ja111009s

The Proapoptotic G41S Mutation to Human Cytochrome c Alters the Heme Electronic Structure and Increases the Electron Self-Exchange Rate, Matthew D. Liptak, Robert D. Fagerlund, Elizabeth C. Ledgerwood, Sigurd M. Wilbanks, Kara L. Bren, J. Am. Chem. Soc. 2011, 133, 1153-1155. DOI: 10.1021/ja106328k

2010

Zinc Porphyrin as a Donor for FRET in Zinc Cytochrome c, Andrea J. Lee, Amy A. Ensign, Todd D. Krauss, and Kara L. Bren, J. Am. Chem. Soc. 2010, 132, 1752-1753. DOI: 10.1021/ja909106p.

NMR and DFT Investigation of Heme Ruffling: Functional Implications for Cytochrome c, Matthew D. Liptak, Xin Wen, and Kara L. Bren, J. Am. Chem. Soc. 2010, 132, 9753–9763. DOI: 10.1021/ja102098p.

A Heme Fusion Tag for Protein Affinity Purification and Quantitation, Wesley B. Asher and Kara L. Bren, Protein Science 2010, DOI: 10.1002/pro.460

Variation and Analysis of Second-sphere Interactions and Axial Histidinate Character in c-type Cytochromes, Sarah E. J. Bowman and Kara L. Bren, Inorg. Chem. 2010, 49, DOI: 10.1021/ic100899k

2009

Studies of Ferric Heme Proteins with Highly Anisotropic/Highly Axial Low Spin (S= 1/2) Electron Paramagnetic Resonance Signals with Two Histidines or Histidine and Methionine Axial Fe Coordination. Giorgio Zoppellaro, Kara L. Bren, Amy A. Ensign, Espen Harbitz, Ravinder Kaur, Hans–Petter Hersleth, Ulf Ryde, Lars Hederstedt, and K. Kristoffer Andersson, Biopolymers 2009, 91, 1064-1082. DOI: 10.1002/bip.21267

 

2008

Modulation of the Ligand-Field Anisotropy in a Series of Ferric Low-Spin Cytochrome c Mutants derived from Pseudomonas aeruginosa Cytochrome c-551 and Nitrosomonas europaea Cytochrome c-552: A Nuclear Magnetic Resonance and Electron Paramagnetic Resonance Study. Giorgio Zoppellaro, Espen Harbitz, Ravinder Kaur, Amy A. Ensign, Kara L. Bren, and K. Kristoffer Andersson, J. Am. Chem. Soc. 2008, 130, 15348-15360. DOI: 10.1021/ja8033312

Methionine Ligand Lability of Type I Cytochromes c: Detection of Ligand Loss Using Protein Film Voltammetry. Tao Ye, Ravinder Kaur, F. Timur Senguen, Lea V. Michel, Kara L. Bren and Sean J. Elliott, J. Am. Chem. Soc. 2008, 130, 6682–6683. DOI: 10.1021/ja801071n

Zinc porphyrin: A fluorescent acceptor in studies of Zn-cytochrome c unfolding by fluorescence resonance energy transfer. Amy A. Ensign, Iris Jo, Ilyas Yildirim, Todd D. Krauss, and Kara L. Bren, Proc. Natl. Acad. Sci. U.S.A. 2008, 105, 10779-10784. DOI: 10.1073/pnas.0802737105

The chemistry and biochemistry of heme c: functional bases for covalent attachment. Sarah E. J. Bowman and Kara L. Bren, Nat. Prod. Rep.2008, 25, 1118-1130. DOI: 10.1039/b717196j

Native and Unfolded Cytochrome c - Comparison of Dynamics using 2D-IR Vibrational Echo Spectroscopy. Seongheun Kim, Jean K. Chung, Kyungwon Kwak, Sarah E. J. Bowman, Kara L. Bren, Biman Bagchi and M. D. Fayer, J. Phys. Chem. B , 2008, 112, 10054-10063. DOI: 10.1021/jp802246h

2007

Heme Attachment Motif Mobility Tunes Cytochrome c Redox Potential. Lea V. Michel, Tao Ye, Sarah E. J. Bowman, Benjamin D. Levin, Megan A. Hahn, Brandy S. Russell, Sean J. Elliott, and Kara L. Bren, Biochemistry 2007, 46, 11753-11760.  DOI: 10.1021/bi701177j

Effects of Heme Pocket Structure and Mobility on Cytochrome c Stability. Xin Wen, Kirti M. Patel, Brandy S. Russell, and Kara L. Bren, Biochemistry 2007, 46,2537-2544. DOI: 10.1021/bi602380v

2006

Cytochrome c₅₅₂ Mutants: Structure and Dynamics at the Active Site Probed by Multidimensional NMR and Vibration Echo Spectroscopy. Aaron M. Massari, Brian L. McClain, Ilya J. Finkelstein, Andrew P. Lee, Heather L. Reynolds, Kara L. Bren, and Michael D. Fayer, J. Phys. Chem. B 2006, 110, 18803-18810. DOI: 10.1021/jp054959q

2005

Redox Properties of Wild-Type and Heme-Binding Loop Mutants of Bacterial Cytochromes c Measured by Direct Electrochemistry. Tao Ye, Ravinder Kaur, Xin Wen, Kara L. Bren, and Sean J. Elliott, Inorg. Chem., 2005, 44, 8999-9006. DOI: 10.1021/ic051003l

Heme Axial Methionine Fluxion in Pseudomonas aeruginosa Asn64Gln Cytochrome c₅₅₁. Xin Wen and Kara L. Bren, Inorg. Chem. 2005, 44, 8587-8593. DOI: 10.1021/ic050976i

The Influence of Aqueous versus Glassy Solvents on Protein Dynamics: Vibrational Echo Experiments and Molecular Dynamics Simulations. Aaron M. Massari, Ilya J. Finkelstein, Brian L. McClain, Anne Goj, Xin Wen, Kara L. Bren, Roger F. Loring, and Michael D. Fayer, J. Am. Chem. Soc. 2005, 127, 14279-14289. DOI: 10.1021/ja053627w

An Obligatory Intermediate in the Folding Pathway of Cytochrome c₅₅₂ from Hydrogenobacter thermophilus. Carlo Travaglini-Allocatelli, Stefano Gianni, Vikash K. Dubey, Allesandro Borgia, Adele Di Matteo, Daniele Bonivento, Francesca Cutruzzolà, Kara L. Bren, and Maurizio Brunori, J. Biol. Chem. 2005, 280, 25729-25734. DOI: 10.1074/jbc.M502628200

Suppression of Axial Methionine Fluxion in Hydrogenobacter thermophilus Gln64Asn-Cytochrome c₅₅₂. Xin Wen and Kara L. Bren, Biochemistry 2005, 44, 5225-5233. DOI: 10.1021/bi047556+.

2004

Folding, Conformational Changes, and Dynamics of Cytochromes c Probed by NMR Spectroscopy. Kara L. Bren, Jason A. Kellogg, Ravinder Kaur, and Xin Wen, Inorg. Chem. 2004, 43, 7934-7944. DOI: 10.1021/ic048925t

Cytochrome rC₅₅₂, Formed During Expression of the Truncated, Thermus thermophilus Cytochrome c₅₅₂ Gene in the Cytoplasm of Escherichia coli, Reacts Spontaneously to Form Protein-Bound, 2-Formyl-4-vinyl (Spirographis) Heme. James A. Fee, Thomas R. Todaro, Eugene Luna, Donita Sanders, Laura Hunsicker-Wang, Kirti M. Patel, Kara L. Bren, Ester Gomez-Moran, Michael G. Hill, Jingyuan Ai, Thomas M. Loehr, W. Anthony Oertling, Pamela A. Williams, C. David Stout, Duncan McRee, and Andrzej Pastuszyn, Biochemistry 2004, 43, 12162-12176. DOI: 10.1021/bi048968l

Heme Axial Methionine Fluxionality in Hydrogenobacter thermophilus Cytochrome c₅₅₂. Linghao Zhong, Xin Wen, Terry M. Rabinowitz, Brandy S. Russell, Elizabeth F. Karan, Kara L. Bren, Proc. Natl. Acad. Sci. U.S.A. 2004, 101, 8637-8642. DOI: 10.1073/pnas.0402033101

2003

Backbone Dynamics and Hydrogen Exchange of Oxidized Pseudomonas aeruginosa Ferricytochrome c₅₅₁. Brandy S. Russell, Linghao Zhong, Maria Giulia Bigotti, Francesca Cutruzzolà, and Kara L. Bren, J. Biol. Inorg. Chem. 2003, 8, 156-166. DOI: 10.1007/s00775-002-0401-z

2002

Characterization of Recombinant Horse Cytochrome c Synthesized with the Assistance of Escherichia coli Cytochrome c Maturation Factors. Jason A. Kellogg and Kara L. Bren, Biochim. Biophys. Acta 2002, 1601, 215-221. DOI: 10.1016/S1570-9639(02)00471-5

Denaturant Dependence of Equilibrium Unfolding Intermediates and Denatured State Structure of Horse Ferricytochrome c.  Brandy S. Russell and Kara L. Bren, J. Biol. Inorg. Chem. 2002, 7, 909-916. DOI: 10.1007/s00775-002-0381-z

Peptide Mimotopes of Pneumococcal Capsular PS of 6B Serotype. A Peptide Mimotope Can Bind to Two Unrelated Antibodies.  Jeon-Soo Shin, Jigui Yu, Jisheng Lin, Linghao Zhong, Kara L. Bren, and Moon H. Nahm., J. Immunol. 2002, 168, 6273-6278. http://www.jimmunol.org/cgi/reprint/168/12/6273

Characterization of Hydrogenobacter thermophilus Cytochromes c₅₅₂ Expressed in the Cytoplasm and Periplasm of Escherichia coli.  Elizabeth F. Karan, Brandy S. Russell and Kara L. Bren, J. Biol. Inorg. Chem. 2002, 7, 260-272. DOI: 10.1007/s007750100292

2001

Recombinant Cytochrome rC₅₅₇ Obtained from Escherichia coli Cells Expressing a Truncated Thermus thermophilus cycA gene: Heme Inversion in an Improperly Matured Protein.  Duncan E. McRee, Pamela A. Williams, Vandana Sridhar, Andrzej Pastuszyn, Kara L. Bren, Kirti M. Patel, Ying Chen, Thomas R. Todaro, Donita Sanders, Eugene Luna, and James A. Fee, J. Biol. Chem. 2001, 276, 6537-6544. DOI: 10.1074/jbc.M008421200

2000

Integrity of Thermus thermophilus Cytochrome c552 Synthesized by Escherichia coli Cells Expressing the Host-Specific Cytochrome c Maturation Genes, ccmABCDEFGH:  Biochemical, Spectral and Structural Characterization of the Recombinant Protein. James A. Fee, Ying Chen, Thomas R. Todaro, Kara L. Bren, Kirti M. Patel, Michael G. Hill, Ester Gomez-Moran, Thomas M. Loehr, Jingyuan Ai, Linda Thöny-Meyer, Pamela A. Williams, Enrico Stura, Vandana Sridhar, and Duncan E. McRee, Protein Sci. 2000, 9, 2074-2084

NMR Investigation of Ferricytochrome c Unfolding: Detection of an Equilibrium Unfolding Intermediate and Residual Structure in the Denatured State.  Brandy S. Russell, Rory Melenkivitz, and Kara L. Bren, Proc. Natl. Acad. Sci. U.S.A. 2000, 97, 8312-8317. DOI: 10.1073/pnas.150239397